Reduction of toxicity of multi-targeting antifolates

ABSTRACT

The use of tetrahydrofolate, methylene-tetrahydrofolate and/or methyl-tetrahydrofolate, and at least one multi-targeting antifolate, for the manufacture of a pharmaceutical composition for the treatment of cancer is disclosed. By combining the multi-targeting antifolate with tetrahydrofolate, methylene-tetrahydrofolate and/or methyl-tetrahydrofolate, it is possible to remarkably reduce toxic side-effects without diminishing the anti-tumour action of the drugs. A pharmaceutical composition, a kit comprising the pharmaceutical composition as well as a method for the treatment of cancer are also disclosed.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation of and claims priority under 35U.S.C. §120 on U.S. patent application Ser. No. 10/583,508 filed on May15, 2007, which is a National Phase of PCT Patent Application No.PCT/SE2004/001955, filed on Dec. 22, 2004, which claims priority under35 U.S.C. §119 to Sweden Patent Application No. 0303526-8, filed on Dec.22, 2003, the contents of each of which are hereby incorporated byreference in their entirety.

TECHNICAL FIELD

The present invention relates to the use of multi-targeting antifolatesfor the manufacture of a pharmaceutical composition for the treatment ofcancer.

BACKGROUND ART

The control of drug-related toxicity (e.g. myelosuppression, diarrhea,mucosal toxicity, and infection) has been a major concern throughout thehistory of cancer chemotherapy. Substances conventionally used in cancerchemotherapy are e.g. antifolates, anthracyclines, and antineoplasticagents.

Antifolates, or folate antagonists, constitute a class ofantimetabolites, and are active chemotherapeutic agents for many solidtumor and hematologic malignancies (Thomas Purcell W et al; “NovelAntifolate Drugs”, Evolving Therapies, pp 114-125, March 2003).

For many years, methotrexate (MTX) has been the major antifolateantimetabolic agent used in clinical medicine. The primary cellulartarget of MTX is the enzyme dihydrofolate reductase (DHFR) and thus, MTXis a single enzyme targeting antifolate. There are also other approvedagents which only attack single enzyme targets, e.g. 5-fluorouracil(5-FU), which inhibit thymidylate synthase (TS) (Scagliotti, Giorgio Vet al; Phase II Study of Pemetrexed With and Without Folic Acid andVitamin B12 as Front-Line Therapy in Malignant Pleural Mesothelioma”,Journal of Clinical Oncology, Vol 21, No 8, Apr. 15, 2003, pp1556-1561).

Although response to treatment is observed in patients, many of themrelapse due to development of resistance. Drug resistance is often alimiting factor in successful chemotherapy with single enzyme targetingantifolates (Banerjee D et al; “Novel aspects of resistance to drugstargeted to dihydrofolate reductase and thymidylate synthase”,Biochimica et Biophysica Acta 1587 (2002) 164-173).

Novel antifolates have been developed to improve the efficacy andtoxicity profile or to decrease the various known mechanisms ofresistance to antifolate therapy.

These novel antifolates are multi-targeting antifolates that havedemonstrated broad-spectrum antitumor activity. This new generation ofantifolates inhibits several key folate-requiring enzymes of thethymidine and purine biosynthetic pathways, in particular thymidylatesynthase, DHFR and GARFT (glycinamide ribonucleotide formyltransferase),by competing with reduced folates for binding sites. The consequentinhibition of intracellular folate metabolism leads to the inhibition ofcell growth (Niyikiza Clet et al; “Homocysteine and Methylmalonic Acid:Markers to Predict and Avoid Toxicity from Pemetrexed Therapy”,Molecular Cancer Therapeutics, Vol 1, 545-552, May 2002).

The cytotoxic activity and subsequent effectiveness of antifolates canbe associated with substantial toxicity for some patients. Antifolates,as a class, have been associated with sporadic severe myelosuppressionwith gastrointestinal toxicity. A combination of such toxicities cancarry a high risk of mortality. The inability to control thesetoxicities has led to the discontinuation of clinical development ofsome antifolates, and complicated the clinical development of others(Niyikiza Clet et al; “Homocysteine and Methylmalonic Acid Markers toPredict and Avoid Toxicity from Pemetrexed Therapy”, Molecular CancerTherapeutics, Vol 1, 545-552, May 2002).

U.S. Pat. No. 5,376,658 (Spears et al) discloses the use of CF₂FH₄, andits solution product isomer FH₄, as a modulator of 5-FU in cancerchemotherapy. Also disclosed is a method of using CF₂FH₄ or FH₄ in orderto reduce the toxicity of an anti-folate drug which has beenadministered to a patient. The anti-folate drugs disclosed aremethotrexate, trimetrexate, nitrous oxide, and dideoxytetrahydrofolicacid, all of which belong to the group of single enzyme targetingantifolates.

Treatment with specific TS inhibitors like 5-FU in combination withfolinic acid has been shown to reduce side-effects without reducingtumor effect. It seems that folate deficiency may have contributed tothe toxicity in some cancer patients, and nutritional supplementationwith folic or folinic acid had led to a reduction in toxicity andtreatment-related deaths with preservation of anticancer activity(Calvert H; “Folate status and the safety profile of antifolates”; SeminOncol 2002; 29:3-7).

In a recent study, Niyikiza et al reported that supplementation withfolic acid may lead to a better safety profile in patients treated withpemetrexed, and possibly to an improved efficacy. Toxicity could bemodulated by folic acid supplementation, and the maximum tolerated dosecould be increased (Niyikiza C et al, “Homocysteine and methylmalonicacid: markers to predict and avoid toxicity from pemetrexed therapy”;Mol Cancer Ther 2002; 1:545-52).

However, the metabolism of folic acid is a very complex process, andmany metabolic steps are required in order to achieve the activesubstances of the folic acid metabolism. Folic acid is the most oxidizedand stable form of folate and must be deconjugated, reduced, andmethylated to be metabolically active in the cell (Kelly G S, “Folates:supplemental forms and therapeutic applications”; Altern Med Rev 1998;3:208-20).

Folic acid supplementation to reduce toxicity is thus actually quiteinefficient, and may additionally lead to unwanted metabolicintermediates. Until now, no satisfactory way of reducing the toxicityof multi-targeting antifolates has been proposed. Considering the verypromising action of antifolates, a possibility to efficiently reducetheir side effects is very much sought-after. There is thus a need for away of reducing toxicity, and at the same time maintining or improvingthe efficiency of multi-targeting antifolates, in order to be able touse them efficiently in clinic.

SUMMARY OF THE INVENTION

An aim of the present invention is to overcome the above-mentioneddrawbacks and provide an improved way of reducing the toxicity, andmaintaining or improving the efficiency, of multi-targeting antifolates.

This aim is achieved by the use of tetrahydrofolate (THF),methylene-tetrahydrofolate (methylene-THF) and/ormethyl-tetrahydrofolate (methyl-THF), and at least one multi-targetingantifolate, for the manufacture of a pharmaceutical composition for thetreatment of cancer.

The use of THF, methylene-THF and/or methyl-THF in combination with amulti-targeting antifolate according to the present inventiondramatically reduces the side effects of multi-targeting antifolatesand, consequently, the therapeutic index is improved. It is thuspossible to administer a higher dose to the tumor without increasing theside-effects, which leads to a better clinical effect.

Preferably, at least 60% by weight of said THF, methyl-THF and/ormethylene-THF is in the form of a biologically active isomer. Thereby,the synergistic effects of the combination with multi-targetingantifolates are optimised.

Said multi-targeting antifolate may be selected from the groupconsisting of premetrexed, raltitrexed, and lometrexol. However, alsoother multi-targeting antifolates, as well as other substances whichpossess the characteristics of multi-targeting antifolates may be used.

The pharmaceutical composition may further comprise at least onechemotheropeutic agent selected from the group consisting ofanthracyclines, platinum derivatives, topoisomerase inhibitors, andantimetabolites. The reduction of severe side-effects obtained by thepresent invention provides for greater combination possibilities.

Said anthracycline may be selected from the group consisting ofdoxorubicin and epirubicin; said platinum derivative may be selectedfrom the group consisting of oxaliplatin, cisplatin, and carboplatin;said topoisomerase inhibitor may be selected from the group consistingof irinotecan and CPT11 (a topoisomerase I-inhibitor); and saidantimetabolite may be selected from the group consisting ofcapecitabine, gemcitabin, UFT and S1. However, also other substancesbelonging to the aforementioned groups of substances may be used.

The THF, methylene-THF and/or methyl-THF, and said multi-targetingantifolate, may be formulated in different pharmaceutical compositions,or in a common pharmaceutical composition. The formulation intodifferent compositions provides for a great administration flexibility.The formulation into a common pharmaceutical composition, on the otherhand, provides for a simple manufacturing process, as well as for asimple way of administration.

Examples of cancers to be treated according to the invention are breastcancer, gastric cancer, gall bladder cancer, bile duct cancer, coloncancer, rectal cancer, liver cancer, pancreatic cancer, head and neckcancer, and mesotheolioma cancer.

The present invention also relates to a pharmaceutical compositioncomprising at least one multi-targeting antifolate and THF,methylene-THF and/or methyl-THF. Preferably, at least 60% by weight ofsaid THF, methyl-THF and/or methylene-THF is in the form of abiologically active isomer. The pharmaceutical composition may furthercomprise at least one chemotherapeutic agent selected from the groupconsisting of anthracyclines, platinum derivatives, topoisomeraseinhibitors, and antimetabolites.

Further, the present invention relates to a kit comprising apharmaceutical composition comprising at least one multi-targetingantifolate and a pharmaceutical composition comprising THF,methylene-THF and/or methyl-THF or isomers thereof. Preferably, at least60% by weight of said THF, methyl-THF and/or methylene-THF is in theform of a biologically active isomer. Such a kit may further comprise apharmaceutical composition comprising a chemotherapeutic agent selectedfrom the group consisting of, anthracyclines, platinum derivatives,topoisomerase inhibitors, and antimetabolites.

The present invention also relates to a method for the treatment ofcancer, comprising administering to a patient a pharmaceutically activeamount of at least one multi-targeting antifolate and a pharmaceuticallyactive amount of THF, methylene-THF and/or methyl-THF. Preferably, atleast 60% by weight of said THF, methyl-THF and/or methylene-THF is inthe form of a biologically active isomer. The method may furthercomprise the administration of a chemotherapeutic agent selected fromthe group consisting of anthracyclines, platinum derivatives,topoisomerase inhibitors, and antimetabolites.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the main metabolic pathways by which folate impact on DNAsynthesis, repair and methylation.

FIG. 2 shows the percent increase of tissue concentration ofmethylenetetrahydrofolate (MTHF) in liver metastases from colorectalcancer in individual patients.

FIG. 3 shows the mean percent increase of tissue concentration ofmethylenetetrahydrofolate (MTHF) in liver metastases from colorectalcancer in individual patients.

FIG. 4 shows the body weights at day 5 and 13 (mean±SEM) of the animalsin example 2.

FIG. 5 shows the tumor weights of the liver tumors (mean±SEM) of theanimals in example 2.

FIG. 6 shows the spleen weights (mean±SEM) of the animals in example 2.

FIG. 7 shows the TS expression levels after intravenous bolus injectionof a multi-targeting antifolate.

FIG. 8 shows the mean gene expression levels of TS after intravenousbolus injection of a multi-targeting antifolate.

DETAILED DESCRIPTION OF THE INVENTION

In the research work leading to the present invention, the inventorssurprisingly found that by co-administering THF, methylene-THF and/ormethyl-THF, and multi-targeting antifolates, it is possible toremarkably reduce toxic side-effects of the multi-targeting antifolateswithout diminishing the anti-tumour action of the drugs.

The present inventors have shown (see example 2) that by combining amulti-targeting antifolate with the natural form of methylene-THF, thedose of the multi-targeting antifolate may be increased significantlywithout increasing toxicity.

Further, other chemotherapeutic agents, such as anthracyclines, platinumderivaties, topoisomerase inhibitors, and antimetabolites, may beadministered in addition thereto, in order to further increase thecytotoxicity in the tumor while keeping the side-effects at a low level.

5,10-methylene-tetrahydrofolate (in the following referred to asmethylene-THF, or CH₂FH₄) is a normal intracellular metabolite of folicacid, for use in thymidylate synthesis by thymidylate synthase (TS). Thesame is true with respect to the polyglutamates of methylene-THF.Methylene-THF is also used by several other enzymes includingCH₂FH₄-reductase, serine hydroxymethylase and Cl-tetrahydrofolatesynthase and CH₂FH₄ dehydrogenase. These interconversions usingmethylene-THF are essential for purine synthesis, amino acid synthesis,and lipid metabolism. Thus, methylene-THF is located at a metabolicbranch point as a substrate for at least 4 different enzymes (Spears etal; U.S. Pat. No. 5,376,658).

As stated above, previous studies have shown that folate status maymodulate the toxicity and efficacy of cancer chemotherapy. The presentinventors have found that the positive effect of folic acid is due tothe increased level of methylene-THF that is formed from folic acid.Higher levels of methylene-THF in the cells leads to a more efficientTS-inhibition, which results in a thymidine-less state and increasesuracil misincorporation in DNA.

Since methylene-THF is the active substance of folic acid metabolism,the use of this endogen folate for reducing toxicity in cancer treatmentis much more favourable than the use of folic acid.

Dietary folates are a mixture of polyglutamated folates that aredigested to monoglutamyl forms by the action of an enzyme anchored tothe small intestinal brush border membrane and expressed by theglutamate carboxypeptidase II gene (GCPII) (Devlin A M et al, “Glutamatecarboxypeptidase II: a polymorphism associated with lower levels ofserum folate and hyperhomocysteinemia”; Hum Mol Genet 2000; 9:2837-44).After deconjugation in the small intestine, folic acid is reduced totetrahydrofolate in the liver. Reduced folates are secreted into thesmall intestine with bile, where they are reabsorbed and distributed toother tissues.

The reduced folate carrier, RFC-1, is the major transporter of reducedfolates into the cells (Sirotnak F M, Tolner B, “Carrier-mediatedmembrane transport of folates in mammalian cells”; Annu Rev Nutr 1999;19:91-122). Intracellularly, reduced folate monoglutamates are convertedto polyglutamates by the enzyme folylpolyglutamate synthase (FPGS)(Shane B, “Folylpolyglutamate synthesis and role in the regulation ofone-carbon metabolism”; Vitam Horm 1989; 45:263-335). The polyglutamatedform of tetrahydrofolate is then further converted to5,10-methylenetetrahydrofolate (methylene-THF), required as a methyldonor in the conversion of dUMP to dTMP (Spears C P et al,“Deoxyuridylate effects on thymidylatesynthase-5-fluorodeoxyuridylate-folate ternary complex formation”;Biochem Pharmacol 1989; 38:2985-93; Spears C P, et al “Thymidylatesynthetase inhibition in malignant tumors and normal liver of patientsgiven intravenous 5-fluorouracil”, Cancer Res 1984; 44:4144-50). Theconversion is catalysed by thymidylate synthase (TS). Methylene-THF isalso the precursor of the metabolically active 5-methyltetrahydrofolate(methyl-THF), utilized in the remethylation of homocysteine. Conversionof methylene-THF to methyl-THF is dependent on the enzymemethylenetetrahydrofolate reductase (MTHFR).

The enzyme γ-glutamyl hydrolase (GGH) catalyzes the degradation ofinter- and intracellular polyglutamates (Galivan J et al, “Glutamylhydrolase pharmacological role and enzymatic characterization”,Pharmacol Ther 2000; 85:207-15). FIG. 1 summarizes the main metabolicpathways by which folate and homocysteine impact on DNA synthesis,repair and methylation. The enzyme MTHFR balances the DNA methylationand DNA synthesis pathways to maintain normal homeostasis. (RFC=reducedfolate carrier; FPGS=folylpolyglutamate synthase; GGH=γ-glutamylhydrolase; TS=thymidylate synthase; THF=tetrahydrofolate;MTHFR=methylene-tetrahydrofolate reductase; MS=methionine synthase;SAM=S-adenosylmethionine; DPD=dihydro-pyrimidine dehydrogenase;B2=riboflavin; B6=pyridoxine; B12=cobalamin.)

As shown, proper functioning of the DNA synthesis and methylationpathways requires riboflavin (vitamin B2), pyridoxine (vitamin B6), andcobalamin (vitamin B12), in addition to folates. Inadequate levels ofany of these metabolites will result in elevated homocysteine levels.Pyridoxine deficiency will also impair the cellular ability to produceglutathione, the master anti-oxidant needed for detoxification of freeradicals and alkylating agent damage.

Oral folates are generally available in two supplemental forms, folicand folinic acid. The biochemical basis for modulation of fluorouracil(FU) activity by folic acid or folinic acid (leucovorin) is elevation ofthe metabolite methylene-THF, which stabilizes the inhibitory ternarycomplex formed between methylene-THF, thymidylate synthase and theactive metabolite of 5-FU. Folinic acid is an immediate precursor ofmethylene-THF, since oral administration of folinic acid bypasses thedeconjugation and reduction steps needed for folic acid. Folinic acidseems to be a more metabolically active form of folate than folic acidand, as a reduced folate, might directly cross the brain-blood barrier.Like folic acid, folinic acid must be methylated to be metabolicallyactive. The methylation step of folic and folinic acid requires adequatelevels of serine and a functional serine hydroxymethyltransferase (SHMT)enzyme (Costi M P, Ferrari S, “Update on antifolate drugs targets”; CurrDrug Targets 2001; 2:135-66). Supplementation with the reduced folatesmethylene-THF and methyl-THF has the advantage of bypassing thismethylation step.

Pharmacogenetics aims to identify individuals predisposed to high riskof toxicity from conventional doses of cancer chemotherapeutic agents.

Interindividual variability in the efficacy and toxicity of drug therapyis associated with polymorphisms in genes encoding drug-metabolizingenzymes, transporters, or drug targets.

The key metabolite methylene-THF is involved directly in biosynthesis ofdTMP, in conversion to 10-formyl-THF for purine synthesis, and inreduction to methyl-THF for methylation of homocysteine to methionine(Kelly G S. Folates: supplemental forms and therapeutic applications.Altern Med Rev 1998; 3:208-20). The conversion of methylene-THF tomethyl-THF requires the enzyme MTHFR.

Aberrations in the distribution of different folates may be caused bythe MTHFR C677T polymorhism (Guinotte C L et al,“Methylenetetrahydro-folate reductase 677C-->T variant modulates folatestatus response to controlled folate intakes in young women”; J Nutr2003; 133:1272-80). Homo- or heterozygosity for the T allele results ina MTHFR enzyme with suboptimal activity and a raise in methylene-THF. Alink between MTHFR polymorphism and tumor response to 5-FU was detectedin a recent study by Cohen et al (Cohen V et al,“Methylenetetrahydrofolate Reductase Polymorphism in Advanced ColorectalCancer: A Novel Genomic Predictor of Clinical Response toFluoropyrimidine-based Chemotherapy”; Clin Cancer Res 2003; 9:1611-5).Patients with CT or TT genotype responded better to the therapy thanpatients with the CC genotype. The MTHFR C677T genotype may also bepredictive of clinical toxicity to raltitrexed (Stevenson J P et al“Phase I clinical and pharmacogenetic trial of irinotecan andraltitrexed administered every 21 days to patients with cancer”; J ClinOncol 2001; 19:4081-7). Homozygotes with the MTHFR C677T polymorphismexperienced less raltitrexed-associated toxicity compared to those withwild-type or heterozygous genotypes.

Conversion of methylene-THF to methyl-THF may be impaired in individualscarrying a MTHFR enzyme with suboptimal activity (Guinotte C L et al“Methylenetetrahydrofolate reductase 677C-->T variant modulates folatestatus response to controlled folate intakes in young women”; J Nutr2003; 133:1272-80). Also, high levels of homocysteine orS-adenosylhomocysteine, as is found in folate deficient individuals, areknown to inhibit the MTHFR enzyme (De Cabo S F et al, “Molecular andcytological evidence of S-adenosyl-L-homocysteine as an innocuousundermethylating agent in vivo”; Cytogenet Cell Genet 1995; 71:187-92).Thus, when choosing between methylene-THF and methyl-THF it might beimportant to take the patients genotype and folate status intoconsideration.

The X-ray repair cross-complementing gene I (XRCC1) protein has animportant function in base excision repair of DNA. Different polymorphicforms of the protein exist, and the Arg399Gln polymorphism has beenassociated with risk of developing CRC as well as with resistance tooxaliplatin/5-FU chemotherapy (Stoehlmacher J et al, “A polymorphism ofthe XRCC1 gene predicts for response to platinum based treatment inadvanced colorectal cancer” Anticancer Res 2001; 21:3075-9). Possibly,the XRCC1 gene may also be inactivated by aberrant methylation or bymutations caused by folate deficiency. Reversal of oxaliplatinresistance could be accomplished using folates like methylene-THF.

Further, low dietary intake or blood concentrations of folate areassociated with an increased risk of colorectal neoplasia. In fact,epidemiologic studies have established an inverse association betweenfolate status and colorectal neoplasia (Kim et al; “Colonic mucosalconcentrations of folate correlate well with blood measurements offolate status in persons with colorectal polyps”, Am J Clin Nutr 1998;68: 866-72).

The use of THF, methylene-THF and/or methyl-THF for treatment of folatedeficiency in colonic mucosa is very advantageous, since the mostefficient folate is thereby administered directly to the site where itis needed the most.

Methylated folates may also reduce severe diarrhea induced bymulti-targeting antifolates by protecting the neural network in theepithelial mucosa.

In connection to the present invention, natural as well as syntheticTHF, methylene-THF and methyl-THF may be used. Further, prodrugs to THF,methylene-THF and/or methyl-THF may be used. THF, methylene-THF andmethyl-THF may exist in several isomeric forms, however, thebiologically active isomers of THF, methylene-THF and methyl-THF,respectively, are preferred according to the present invention. As usedherein, “biologically active isomer” relates to an endogen isomer ofTHF, methylene-THF or methyl-THF, i.e. a natural form which fit into theenzyme systems in the body.

One natural, biologically active isomer of THF is [6S]-THF, one natural,biologically active isomer of methylene-THF is [6R]-methylene-THF, andone natural, biologically active isomer of methyl-THF is[6S]-methyl-THF.

The present inventors have surprisingly found that the biologicallyactive isomers are most suitable to be used in combination withmulti-targeting antifolates.

It has previously been shown that the natural isomer, i.e. thebiologically active form, of methylene-THF (100% 6R-configuration) is abetter growth factor than the racemic mixture (50% 6R-configuration and50% 6S-configuration). One would therefore expect the natural isomer tobe unfavourable to combine with a chemotherapeutic drug due to the riskfor an excessive growth of the tumor. However, upon combination with amulti-targeting antifolate, the property of being a better growth factoris actually useful, because more cancer cells are then stimulated toenter the cell cycle. This is in this case advantageous, since themulti-targeting antifolates mainly affect cells during cell division.

According to the present invention, it is preferred that at least 60% byweight of the THF, methyl-THF and/or methylene-THF is in the form of abiologically active isomer. More preferably, at least 75% is in the formof a biologically active isomer, and most preferably, at least 95% is inthe form of a biologically active isomer. The higher weight portion of abiologically active isomer, the better result will be achieved. This isthe case for all embodiments of the invention.

THF, methylene-THF and methyl-THF for use according to the inventionsuitably has a purity of 90% or higher. A purity of 97% or higher ispreferred. A higher purity provides for a better stability of thefolates. At low purities, THF, methylene-THF and methyl-THF are verysusceptible to oxidation, and thus unstable.

Alternative expressions for methylene-THF are 5,10-methylene-THF, or5,10-CH₂—FH₄. Methylene-THF may e.g. be used in the form of its Ca orNa₂ salt, or in the form of various sulfate salts. Further, according tothe present invention, the free acid may be used, i.e.methylene-tetrahydrofolic acid, or5,10-methylenetetrahydropteroyl-glutamic acid (5,10-CH₂—H₄—PteGlu).

Alternative expressions for methyl-THF are 5-methyl-THF, or 5-CH₃—H₄F.Methyl-THF may e.g. be used in the form of its Ca or Na₂ salt, or in theform of various sulfate salts. Further, according to the presentinvention, the free acid may be used, i.e. methyl-tetrahydrofolic acid,or methyltetrahydropteroyl-glutamic acid (5-CH₃—H₄PteGlu).

Alternative expressions for THF are H₄F, or 5,6,7,8-tetrahydrofolate.THF may e.g. be used in the form of its Ca or Na₂ salt, or in the formof various sulfate salts. Further, according to the present invention,the free acid may be used, i.e. tetrahydrofolic acid, ortetrahydropteroyl-glutamic acid (H₄PteGlu).

The term “chemotherapeutic agent” as used herein relates to a medicamentfor the treatment of cancer.

The term “multi-targeting antifolate” (or “multi-functional antifolate”)as used herein relates to an antifolate which acts on two or more of theenzymes involved in folate synthesis. The difference between singleenzyme targeting antifolates and multi-targeting antifolates isillustrated in “Pemetrexed Disodium: A Novel Antifolate ClinicallyActive Against Multiple Solid Tumors” by Hanauske A, et al, where theinhibitory activity of pemetrexed and MTX against different enzymes iscompared. Pemetrexed demonstrate significant inhibitory activity formultiple enzyme systems, unlike MTX.

Further, the multifunctionality of the multi-targeting antifolatesdecreases the risk for the development of drug resistance, which is agreat difference compared to single enzyme targeting antifolates.

The multi-targeting antifolate may for example be selected from thegroup consisting of premetrexed (trade name: Alimta), raltitrexed (tradename: Tomudex), and lometrexol. (The multifunctionality of raltitrexedis shown e.g. by Jackman A L et al in “ICI D1694, a quinazolineantifolate thymidylate synthase inhibitor that is a potent inhibitor ofL1210 tumor cell growth in vitro and in vivo: a new agent for clinicalstudy”, Cancer Res 1991 Oct. 15; 51(20):5579-86.)

The terms “anthracycline”, “platinum derivative”, “topoisomeraseinhibitor”, and “antimetabolite” as used herein relates to thesecompounds as defined in the National Library of Medicine.

The term “patient” as used herein relates to any human or non-humanmammal in need of being treated with the methods, kit or pharmaceuticalcompositions according to the invention.

The term “treatment” as used herein relates to both treatment in orderto cure or alleviate the symptoms of different types of cancer, and totreatment in order to prevent the development of cancer. In particular,solid tumors are well suited to be treated according to the invention.

The term “pharmaceutically active amount” as used herein relates to adose of a substance that will lead to the desired pharmacological and/ortherapeutic effect. The desired pharmacological and/or therapeuticeffect is, as stated above, to cure or alleviate the symptoms ofdifferent types of cancer, and to prevent the development of cancer.

THF, methylene-THF and/or methyl-THF, and the multi-targetingantifolate, may be administered simultaneously or consequtively. Whenadministered consequtively, either THF, methylene-THF and/or methyl-THFis administered first and thereafter the multi-targeting antifolate, orthe multi-targeting antifolate is administered first and thereafter THF,methylene-THF and/or methyl-THF. The interval between theadministrations depends on the drug characteristics, and may e.g. varyfrom hours to days. However, shorter and longer intervals may be used.

In case other chemotherapeutic agents, i.e. anthracyclines, platinumderivatives, topoisomerase inhibitors, and antimetabolites, are to beadministered as well, they may be administered either simultaneouslywith THF, methylene-THF and/or methyl-THF, or simultaneously with themulti-functional antifolate. Further, all substances, i.e. THF,methylene-THF and/or methyl-THF, the multi-targeting antifolate, andother chemotherapeutic substances may be administered simultaneously.The other chemotherapeutic agents may also be administered separate fromTHF, methylene-THF and/or methyl-THF, and the multi-targetingantifolate.

The order in which drugs and folate are administered could be of majorimportance for the outcome of chemotherapeutic treatment. As anillustration, Raghunathan et al., showed that tumor growth wassuppressed approximately 80% when 5-FU was administered to folatedepleted animals 1 hr after folinic acid administration, compared withapproximately 50% suppression in control mice (Raghunathan K et al“Impact of schedule on leucovorin potentiation of fluorouracil antitumoractivity in dietary folic acid deplete mice”; Biochem Pharmacol 1997;53:1197-202). Folinic acid administered 12 hr before 5-FU resulted intumor growth stimulation that was consistent with the pronounced growthstimulation when folinic acid was administered without 5-FU.

The pharmaceutical compositions according to the invention may alsocomprise other substances, such as an inert vehicle, or pharmaceuticalacceptable adjuvants, carriers, preservatives, ascorbic acid, ascorbate,antioxidants, etc, which are well known to persons skilled in the art.

The pharmaceutical compositions according to the invention may beformulated by conventional manufacturing methods, such as e.g. bymanufacturing methods similar to those used for the production ofleucovorin.

Examples of cancers to be treated according to the invention are breastcancer, gastric cancer, gall bladder cancer, bile duct cancer, coloncancer, rectal cancer, liver cancer, pancreatic cancer, head and neckcancer, and mesotheolioma cancer.

THF, methylene-THF and/or methyl-THF is preferably administered in adose of 100 μg to 1000 mg, preferably a dose of 100-200 mg,corresponding to approximately 1-5 mg/kg body weight. The dose will beadjusted individually, and may thus vary, e.g. depending on thecondition of the patient.

The dose may be administered e.g. daily, weekly, or monthly. It may, forexample, be administered subcutaneously, intramuscularely,intravenously, intraarterially, intraperitoneally, intranasally ororally.

Furthermore, it is possible to combine the treatment according to theinvention with other conventional pharmacological treatments of cancer.THF, methylene-THF and/or methyl-THF and the multi-targeting antifolatemay thus be administered in combination with other conventionalpharmaceuticals used to treat cancer.

The compositions according to the invention may also be co-administeredwith vitamin B12, vitamin B6, and vitamin B2.

Examples of combinations of multi-targeting antifolates and THF,methylene-THF and/or methyl-THF are:

-   -   Premetrexed+THF, methylene-THF and/or methyl-THF    -   Premetrexed+5-fluorouracil+THF, methylene-THF and/or methyl-THF    -   Raltitrexed+THF, methylene-THF and/or methyl-THF    -   Raltitrexed+5-fluorouracil+THF, methylene-THF and/or methyl-THF.

EXAMPLES

The invention will now be further explained in the following examples.These examples are only intended to illustrate the invention and shouldin no way be considered to limit the scope of the invention.

Example 1 Administration of Methylene-THF Vs Administration of FolinicAcid

The level of increase of tissue concentration of methylene-THF afteradministration of leucovorin and methylene-THF, respectively, has beencompared.

Patients operated upon due to liver metastases were given a dose offolates. Biopses were taken from the tumor before i.v. bolus injectionof leucovorin on methylenetetrahydrofolate. At 20 minutes a new tumorbiopsy was taken and the concentration of MTHF was assayed. Theadministration of methylene-THF led to a greater tissue concentration ofmethylene-THF than leucovorin. The results are shown in FIGS. 2 and 3.These results indicate that administration of methylene-THF instead offolinic acid is more efficient.

Example 2 Multi-Targeting Antifolate Therapy (ALIMTA) in Combinationwith the Natural Isomer of Methylenetetrahydrofolate (R-5,10-CH₂FH₄) inan Experimental Adenocarcinoma in Rats Method

The following experimental study was approved by the local ethicalcommittee at the Göteborg University.

Eight inbred female Wistar rats were during general anesthesiainoculated in the central liver lobe with 0.1 ml of 1.0 million viabletumor cells of an experimental adenocarcinoma. On day 5 the animals wererelaparotomized during general anesthesia, the body weight was recordedand the size of the solitary liver tumor was measured with verniercalipers.

The animals were randomly allocated to two groups. Group 1 receivedintravenous injections of ALIMTA (premetrexed) 0.01 mg/kg and group 2received 0.1 mg/kg of ALIMTA. Both groups received 15 mg/kg ofR-5,10-CH₂FH₄ immediately following the injections of ALIMTA. Therapywas administered through intravenous injections via the tail vein ondays 6-8 and days 11-12. On day 13 all animals were sacrificed by anoverdose of anesthesia.

The body weights were recorded and the size of the liver tumors wasmeasured. The wet weights of the excised liver tumor and spleen wererecorded.

Results

All animals survived the experimental period and no signs of toxicitywere observed. The body weights on day 5 and 13 are shown in FIG. 4.(FIG. 4 shows the body weights at day 5 and 13. Group 1 (day 5=1 and day13=2) and group 2 (day 5=4 and day 13=5). Mean±SEM.)

All animals receiving 0.01 mg/kg of ALIMTA were found with extrahepatictumor growth in the abdominal cavity as metastases to the lungs. Thetumor weights of the liver tumors in both groups are shown in FIG. 5.The total tumor weights of extrahepatic tumorgrowth in the abdominalcavity and lung metastases were not recorded. Only liver tumor weightsare shown in FIG. 5. (FIG. 5 shows the tumor weights of the liver tumorsin group 1 (1) and group 2 (2). Mean±SEM.)

The wet weights of the spleen in the two groups are shown in FIG. 6.(FIG. 6 shows the spleen weights in group 1 (1) and group 2 (2).Mean±SEM.) It is well known in the art that the toxicity of a drug canbe monitored by looking at the spleen weight as a surrogate marker.

CONCLUSION

This experiment is a proof for the principle that it is possible tocombine a multifunctional antifolate with the natural form ofmethylenetetrahydrofolate, and that such combination can improve theantitumour effect. In the group with the highest Alimta dose, noextrahepatic metastasies were recorded, and still no general toxicitycould be seen.

Example 3 The Effect of a Multi-Targeting Antifolate

In this experiment, the effect of a multi-targeting antifolate, i.e.Tomudex (raltitrexed), was shown. FIG. 7 shows the TS expression levelsin the tumor after Tomudex intravenous bolus injection, and FIG. 8 showsthe gene expression levels of TS after Tomudex intravenous bolusinjection.

Nine colorectal cancer patients were included in the study, and theexpression levels were measured in actin, which is a protein serving asan important structural molecule for the cytoskeletons of manyeukaryotic cells.

This experiment shows that by using a multi-targeting antifolate only,the normal mucosa is actually more adversely affected than the tumor.This also corresponds to the clinical findings; severe toxic effectsfrom the bowel made it impossible to continue the treatment.

Thus, the effects obtained by the present invention, i.e. the reductionof the toxicity of multi-functional antifolates while maintaining thecytotoxic effect, are very much sought after.

While the invention has been described in detail and with reference tospecific embodiments thereof, it will be apparent for one skilled in theart that various changes and modifications can be made therein withoutdeparting from the spirit and scope thereof.

1. Use of methylene-tetrahydrofolate and at least one multi-targetingantifolate, for the manufacture of a pharmaceutical composition for thetreatment of cancer.
 2. Use according to claim 1, wherein at least 60%by weight of said methylene-THF is in the form of a biologically activeisomer.
 3. Use according to claim 1, wherein said multi-targetingantifolate is selected from the group consisting of pemetrexed,raltitrexed, and lometrexol.
 4. Use according to claim 1, wherein saidpharmaceutical composition further comprises at least onechemotherapeutic agent selected from the group consisting ofanthracyclines, platinum derivatives, topoisomerase inhibitors, andantimetabolites.
 5. Use according to claim 1, wherein saidmethylene-tetrahydrofolate and said at least one multi-targetingantifolate, are formulated in different pharmaceutical compositions. 6.Use according to claim 1, wherein said methylene-tetrahydrofolate andsaid at least one multi-targeting antifolate, are formulated in a commonpharmaceutical composition.
 7. Use according to claim 1, wherein saidcancer is selected from the group consisting of breast cancer, gastriccancer, gall bladder cancer, bile duct cancer, colon cancer, rectalcancer, liver cancer, pancreatic cancer, head and neck cancer, andmesotheolioma cancer.
 8. A pharmaceutical composition comprisingmethylene-tetrahydrofolate and at least one multi-targeting antifolate.9. A pharmaceutical composition according to claim 8, wherein at least60% by weight of said methylene-THF is in the form of a biologicallyactive isomer.
 10. A pharmaceutical composition according to claim 8,wherein said multi-targeting antifolate is selected from the groupconsisting of pemetrexed, raltitrexed, and lometrexol.
 11. Apharmaceutical composition according to claim 8, further comprising atleast one chemotherapeutic agent selected from the group consisting ofanthracyclines, platinum derivatives, topoisomerase inhibitors, andantimetabolites.
 12. A pharmaceutical composition according to claim 8,wherein said methylene-tetrahydrofolate and said at least onemulti-targeting antifolate, are formulated in different pharmaceuticalcompositions.
 13. A pharmaceutical composition according to claim 8,wherein said methylene-tetrahydrofolate and said at least onemulti-targeting antifolate, are formulated in a common pharmaceuticalcomposition.
 14. A kit comprising a pharmaceutical composition includingmethylene-tetrahydrofolate and a pharmaceutical composition including atleast one multi-targeting antifolate.
 15. A kit according to claim 14,wherein at least 60% by weight of said methylene-tetrahydrofolate is inthe form of a biologically active isomer.
 16. A kit according to claim14, further comprising a pharmaceutical composition including at leastone chemotherapeutic agent selected from the group consisting ofanthracyclines, platinum derivatives, topoisomerase inhibitors, andantimetabolites.
 17. A method for the treatment of cancer comprisingadministering to a patient a pharmaceutically active amount ofmethylene-tetrahydrofolate and a pharmaceutically active amount of atleast one multi-targeting antifolate.
 18. A method according to claim17, wherein at least 60% by weight of said methylene-tetrahydrofolate isin the form of a biologically active isomer.
 19. A method according toclaim 17, wherein said multi-targeting antifolate is selected from thegroup consisting of pemetrexed, raltitrexed, and lometrexol.
 20. Amethod according to claim 17, further comprising administering apharmaceutically active amount of a chemotherapeutic agent selected fromthe group consisting of anthracyclines, platinum derivatives,topoisomerase inhibitors, and antimetabolites.
 21. A method according toclaim 17, wherein said methylene-tetrahydrofolate and said at least onemulti-targeting antifolate, are administered consecutively.
 22. A methodaccording to claim 17, wherein said methylene-tetrahydrofolate and saidat least one multi-targeting antifolate, are administeredsimultaneously.
 23. A method according to claim 17, wherein said canceris selected from the group consisting of breast cancer, gastric cancer,gall bladder cancer, bile duct cancer, colon cancer, rectal cancer,liver cancer, pancreatic cancer, head and neck cancer, and mesotheoliomacancer.
 24. Use according to claim 1, wherein saidmethylene-tetrahydrofolate is [6R]-methylene-tetrahydrofolate.
 25. Apharmaceutical composition according to claim 8, wherein saidmethylene-tetrahydrofolate is [6R]-methylene-tetrahydrofolate.
 26. A kitaccording to claim 14, wherein said methylene-tetrahydrofolate is[6R]-methylene-tetrahydrofolate.
 27. A method according to claim 17,wherein said methylene-tetrahydrofolate is[6R]-methylene-tetrahydrofolate.